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Effect of acute noise trauma on the gene expression profile of the hippocampus.
Lee, Chang Ho; Kim, Kyung Woon; Lee, So Min; Kim, So Young.
Afiliação
  • Lee CH; Department of Otorhinolaryngology, CHA University College of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, Gyeonggi-do, Korea.
  • Kim KW; Department of Otorhinolaryngology, CHA University College of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, Gyeonggi-do, Korea.
  • Lee SM; Department of Otorhinolaryngology, CHA University College of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, Gyeonggi-do, Korea.
  • Kim SY; Department of Otorhinolaryngology, CHA University College of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, Gyeonggi-do, Korea. sossi81@hanmail.net.
BMC Neurosci ; 21(1): 45, 2020 11 07.
Article em En | MEDLINE | ID: mdl-33160313
BACKGROUND: This study aimed to investigate the changes in the expression of hippocampal genes upon acute noise exposure. METHODS: Three-week-old Sprague-Dawley rats were assigned to control (n = 15) and noise (n = 15) groups. White noise (2-20 kHz, 115 dB sound pressure level [SPL]) was delivered for 4 h per day for 3 days to the noise group. All rats were sacrificed on the last day of noise exposure, and gene expression in the hippocampus was analyzed using a microarray. Pathway analyses were conducted for genes that showed differential expression ≥ 1.5-fold and P ≤ 0.05 compared to the control group. The genes included in the putative pathways were measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). RESULTS: Thirty-eight upregulated genes and 81 downregulated genes were identified. The pathway analyses revealed that upregulated genes were involved in the cellular responses to external stimuli and immune system pathways. qRT-PCR confirmed the upregulation of the involved genes. The downregulated genes were involved in neuronal systems and synapse-related pathways, and qRT-PCR confirmed the downregulation of the involved genes. CONCLUSIONS: Acute noise exposure upregulated the expression of immune-related genes and downregulated the expression of neurotransmission-related genes in the hippocampus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ferimentos e Lesões / Hipocampo / Ruído Limite: Animals Idioma: En Revista: BMC Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ferimentos e Lesões / Hipocampo / Ruído Limite: Animals Idioma: En Revista: BMC Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2020 Tipo de documento: Article