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Profiling Microglia From Alzheimer's Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue.
Alsema, Astrid M; Jiang, Qiong; Kracht, Laura; Gerrits, Emma; Dubbelaar, Marissa L; Miedema, Anneke; Brouwer, Nieske; Hol, Elly M; Middeldorp, Jinte; van Dijk, Roland; Woodbury, Maya; Wachter, Astrid; Xi, Simon; Möller, Thomas; Biber, Knut P; Kooistra, Susanne M; Boddeke, Erik W G M; Eggen, Bart J L.
Afiliação
  • Alsema AM; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Jiang Q; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Kracht L; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Gerrits E; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Dubbelaar ML; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Miedema A; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Brouwer N; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Hol EM; Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands.
  • Middeldorp J; Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands.
  • van Dijk R; Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands.
  • Woodbury M; Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States.
  • Wachter A; Neuroscience Discovery, AbbVie Deutschland GmbH and Co. KG, Ludwigshafen, Germany.
  • Xi S; Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States.
  • Möller T; Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States.
  • Biber KP; Neuroscience Discovery, AbbVie Deutschland GmbH and Co. KG, Ludwigshafen, Germany.
  • Kooistra SM; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Boddeke EWGM; Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
  • Eggen BJL; Department of Cellular and Molecular Medicine, Center for Healthy Ageing, University of Copenhagen, Copenhagen, Denmark.
Front Mol Neurosci ; 13: 134, 2020.
Article em En | MEDLINE | ID: mdl-33192286
ABSTRACT
Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer's disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell levels in non-demented elderly and AD donors using acute human postmortem cortical brain samples. We identified seven human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Mol Neurosci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Holanda
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Mol Neurosci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Holanda