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Elevated Alu retroelement copy number among workers exposed to diesel engine exhaust.
Wong, Jason Y Y; Cawthon, Richard; Dai, Yufei; Vermeulen, Roel; Bassig, Bryan A; Hu, Wei; Duan, Huawei; Niu, Yong; Downward, George S; Leng, Shuguang; Ji, Bu-Tian; Fu, Wei; Xu, Jun; Meliefste, Kees; Zhou, Baosen; Yang, Jufang; Ren, Dianzhi; Ye, Meng; Jia, Xiaowei; Meng, Tao; Bin, Ping; Hosgood Iii, H Dean; Silverman, Debra T; Rothman, Nathaniel; Zheng, Yuxin; Lan, Qing.
Afiliação
  • Wong JYY; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA jason.wong@nih.gov.
  • Cawthon R; Department of Human Genetics, University of Utah, Salt Lake City, Utah, USA.
  • Dai Y; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Vermeulen R; Division of Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands.
  • Bassig BA; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
  • Hu W; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
  • Duan H; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Niu Y; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Downward GS; Division of Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands.
  • Leng S; Department of Internal Medicine, School of Medicine, University of New Mexico, Albuquerque, New Mexico, USA.
  • Ji BT; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
  • Fu W; Chaoyang Center for Disease Control and Prevention, Chaoyang, Liaoning, China.
  • Xu J; Hong Kong University, Hong Kong, China.
  • Meliefste K; Division of Environmental Epidemiology, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands.
  • Zhou B; China Medical University, Shenyang, Liaoning, China.
  • Yang J; Chaoyang Center for Disease Control and Prevention, Chaoyang, Liaoning, China.
  • Ren D; Chaoyang Center for Disease Control and Prevention, Chaoyang, Liaoning, China.
  • Ye M; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Jia X; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Meng T; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Bin P; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Hosgood Iii HD; Division of Epidemiology, Albert Einstein College of Medicine, Yeshiva University, New York, New York, USA.
  • Silverman DT; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
  • Rothman N; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
  • Zheng Y; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
  • Lan Q; Occupational and Environmental Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, Maryland, USA.
Occup Environ Med ; 78(11): 823-828, 2021 11.
Article em En | MEDLINE | ID: mdl-34039759
BACKGROUND: Millions of workers worldwide are exposed to diesel engine exhaust (DEE), a known genotoxic carcinogen. Alu retroelements are repetitive DNA sequences that can multiply and compromise genomic stability. There is some evidence linking altered Alu repeats to cancer and elevated mortality risks. However, whether Alu repeats are influenced by environmental pollutants is unexplored. In an occupational setting with high DEE exposure levels, we investigated associations with Alu repeat copy number. METHODS: A cross-sectional study of 54 male DEE-exposed workers from an engine testing facility and a comparison group of 55 male unexposed controls was conducted in China. Personal air samples were assessed for elemental carbon, a DEE surrogate, using NIOSH Method 5040. Quantitative PCR (qPCR) was used to measure Alu repeat copy number relative to albumin (Alb) single-gene copy number in leucocyte DNA. The unitless Alu/Alb ratio reflects the average quantity of Alu repeats per cell. Linear regression models adjusted for age and smoking status were used to estimate relations between DEE-exposed workers versus unexposed controls, DEE tertiles (6.1-39.0, 39.1-54.5 and 54.6-107.7 µg/m3) and Alu/Alb ratio. RESULTS: DEE-exposed workers had a higher average Alu/Alb ratio than the unexposed controls (p=0.03). Further, we found a positive exposure-response relationship (p=0.02). The Alu/Alb ratio was highest among workers exposed to the top tertile of DEE versus the unexposed controls (1.12±0.08 SD vs 1.06±0.07 SD, p=0.01). CONCLUSION: Our findings suggest that DEE exposure may contribute to genomic instability. Further investigations of environmental pollutants, Alu copy number and carcinogenesis are warranted.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Emissões de Veículos / Exposição Ocupacional / Elementos Alu / Poluentes Ocupacionais do Ar Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Humans / Male / Middle aged Idioma: En Revista: Occup Environ Med Assunto da revista: MEDICINA OCUPACIONAL / SAUDE AMBIENTAL Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Emissões de Veículos / Exposição Ocupacional / Elementos Alu / Poluentes Ocupacionais do Ar Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Humans / Male / Middle aged Idioma: En Revista: Occup Environ Med Assunto da revista: MEDICINA OCUPACIONAL / SAUDE AMBIENTAL Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos