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Single-cell RNA sequencing reveals atlas of dairy goat testis cells.
Yu, Xiu-Wei; Li, Tong-Tong; Du, Xiao-Min; Shen, Qiao-Yan; Zhang, Meng-Fei; Wei, Yu-Dong; Yang, Dong-Hui; Xu, Wen-Jing; Chen, Wen-Bo; Bai, Chun-Ling; Li, Xue-Ling; Li, Guang-Peng; Li, Na; Peng, Sha; Liao, Ming-Zhi; Hua, Jin-Lian.
Afiliação
  • Yu XW; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Li TT; College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
  • Du XM; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Shen QY; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Zhang MF; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Wei YD; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Yang DH; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Xu WJ; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Chen WB; College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China.
  • Bai CL; Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
  • Li XL; Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
  • Li GP; Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot, 010021, China.
  • Li N; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China.
  • Peng S; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China. E-mail:pengshacxh@nwsuaf.edu.cn.
  • Liao MZ; College of Life Science, Northwest A & F University, Yangling, Shaanxi 712100, China. E-mail:liaomz@nwsuaf.edu.cn.
  • Hua JL; College of Veterinary Medicine, Northwest A & F University, Shaanxi Centre of Stem Cells Engineering & Technology, Yangling, Shaanxi 712100, China. E-mail:jinlianhua@nwsuaf.edu.cn.
Zool Res ; 42(4): 401-405, 2021 Jul 18.
Article em En | MEDLINE | ID: mdl-34047080
Single-cell RNA sequencing (scRNA-seq) is useful for exploring cell heterogeneity. For large animals, however, little is known regarding spermatogonial stem cell (SSC) self-renewal regulation, especially in dairy goats. In this study, we described a high-resolution scRNA-seq atlas derived from a dairy goat. We identified six somatic cell and five spermatogenic cell subtypes. During spermatogenesis, genes with significantly changed expression were mainly enriched in the Notch, TGF-ß, and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency. We detected and screened specific candidate marker genes ( TKTL1 and AES) for spermatogonia. Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Cabras / Análise de Sequência de RNA / Análise de Célula Única Limite: Animals Idioma: En Revista: Zool Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testículo / Cabras / Análise de Sequência de RNA / Análise de Célula Única Limite: Animals Idioma: En Revista: Zool Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China