MiRNA-199a-5p targets WNT2 to regulate depression through the CREB/BDNF signaling in hippocampal neuron.
Brain Behav
; 11(8): e02107, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-34333859
ABSTRACT
INTRODUCTION:
This study mainly investigated the role of miR-199a-5p in depression.METHODS:
qRT-PCR and western blotting were employed to detect the expressions of miR-199a-5p, CREB and BDNF. Sucrose preference test, forced swimming test, and tail suspension test were performed to evaluate depression-related symptoms. MTT assays and flow cytometry were used to examine the cell reproduction and apoptotic cells of hippocampal neuron.RESULTS:
The data demonstrated that the expression levels of miR-199a-5p in the cerebrospinal fluids and serums of depression patient and the hippocampus of chronic unpredictable mild stress (CUMS) mouse were significantly increased. However, the expressions of WNT2, p-CREB, and BDNF were inhibited. In addition, miR-199a-5p-inhibitor enhanced sucrose preferences of CUMS mouse and decreased immobile time in sucrose preference test and forced swimming test. Knockdown of WNT2 attenuated the effects of miR-199a-5p-inhibitor on cell reproduction and apoptotic cells of hippocampal neuron and the expression of WNT2, p-CREB, and BDNF.CONCLUSION:
MiR-199a-5p can target WNT2 to enhance the development of depression through regulation of the CREB/BDNF signaling. TRIAL REGISTRATION JNU-Hos-49284.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Brain Behav
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China