Determining the efficacy of the bovine amniotic membrane homogenate during the healing process in rabbits' ex vivo corneas.
Vet Ophthalmol
; 24(4): 380-390, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-34402564
ABSTRACT
OBJECTIVE:
To evaluate the efficacy of bovine amniotic membrane homogenate (BAMH) on wounded ex vivo rabbit corneas. PROCEDURE Eighteen corneas obtained from normal rabbit eyes were wounded equally using a 6 mm trephine and cultured into an air-liquid interface model. Corneas were treated with phosphate-buffered saline (PBS) (n = 6, control group), 0.2% ethylenediaminetetraacetic acid (EDTA; n = 6), or BAMH (n = 6). All treatments were applied topically 6 times/day. Each cornea was macrophotographed daily with and without fluorescein stain to assess epithelialization and haziness. After 7 days, corneal transparency was evaluated, and the tissues prepared for histologic analysis of viability, and total and epithelial thickness.RESULTS:
The mean epithelialization time was 6.2 ± 0.82 days for the control group, 6.2 ± 0.75 days for the EDTA-treated group, and 5.1 ± 0.40 days for the BAMH-treated group, demonstrating a significant difference between the BAMH and the other groups. The corneas that received EDTA had better transparency compared with the other groups. Histologically, all corneas had adequate morphology and architecture after healing. Analysis of corneal and epithelial thickness revealed no significant difference among groups.CONCLUSION:
Bovine amniotic membrane homogenate is an effective and promising treatment for stromal and epithelial ulcers.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Lesões da Córnea
/
Âmnio
Limite:
Animals
Idioma:
En
Revista:
Vet Ophthalmol
Assunto da revista:
MEDICINA VETERINARIA
/
OFTALMOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos