The Effects of Trypsin Inhibitor on Insulin Secretion Using Rat Pancreas in an Organ Bath.
In Vivo
; 35(5): 2551-2558, 2021.
Article
em En
| MEDLINE
| ID: mdl-34410942
ABSTRACT
BACKGROUND/AIM:
We developed an experimental method to reproduce insulin secretion from isolated rat pancreas preparations using an organ bath system. However, secretion of trypsin, another pancreatic enzyme, interferes with insulin production in such systems. We aimed to ascertain the minimum trypsin inhibitor (TI), dose for obtaining a sustained, stable rate of insulin secretion. MATERIALS ANDMETHODS:
The action of TI (1-10 µg/ml) on pancreatic preparations of male Wistar-Imamichi rats in organ bath experiments was assessed by measuring insulin, amylase, and trypsin activity.RESULTS:
The level of insulin outflow remained steady in the TI-treated samples, in contrast to that in the untreated control, where insulin secretion decreased over time. The level of amylase outflow did not change significantly. Trypsin activity was significantly lower in the TI-treated samples than in the control.CONCLUSION:
Even low concentrations of TI can maintain insulin secretion by inhibiting trypsin activity in organ bath experiments.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores da Tripsina
/
Amilases
Limite:
Animals
Idioma:
En
Revista:
In Vivo
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão