Nanoparticle-eluting stents for coronary intervention: formulation, characterization, and in vitro evaluation.
Can J Physiol Pharmacol
; 100(3): 220-233, 2022 Mar.
Article
em En
| MEDLINE
| ID: mdl-34570985
ABSTRACT
Coronary artery disease (CAD) is currently a leading cause of death worldwide. In the history of percutaneous coronary intervention for the treatment of CAD, a drug-eluting stent (DES) is recognized as a revolutionary technology that has the unique ability to significantly reduce restenosis and provide both mechanical and biological solutions simultaneously to the target lesion. The aim of the research work was to design and fabricate DES coated with a nanoparticulate drug formulation. Sirolimus, an inhibitor of the smooth muscle cell (SMC) proliferation and migration, was encapsulated in polymeric nanoparticles (NPs). The NP formulation was characterized for various physicochemical parameters. Cell viability and cell uptake studies were performed using human coronary artery smooth muscle cells (HCASMCs). The developed NP formulation showed enhanced efficacy compared to plain drug solution and exhibited time-dependent uptake into the HCASMCs. The developed NP formulation was coated on the Flexinnium™ ultra-thin cobalt-chromium alloy coronary stent platform. The NP-coated stents were characterized for morphology and residual solvent analysis. In vitro drug release was also evaluated. Ex vivo arterial permeation was carried out to evaluate the NP uptake from the surface of the stents. The characterization studies together corroborated that the developed NP coated stent can be a promising replacement of the current DESs.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sirolimo
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Composição de Medicamentos
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Nanopartículas
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Stents Farmacológicos
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Intervenção Coronária Percutânea
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Liberação Controlada de Fármacos
Limite:
Humans
Idioma:
En
Revista:
Can J Physiol Pharmacol
Ano de publicação:
2022
Tipo de documento:
Article