Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons.

Wimmer, Franziska; Mougiakos, Ioannis; Englert, Frank; Beisel, Chase L

Wimmer, Franziska; Mougiakos, Ioannis; Englert, Frank; Beisel, Chase L.

Afiliação

Wimmer F; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Mougiakos I; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Englert F; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany.
Beisel CL; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany; Medical Faculty, University of Würzburg, 97080 Würzburg, Germany. Electronic address: chase.beisel@helmholtz-hiri.de.

Mol Cell ; 82(6): 1210-1224.e6, 2022 03 17.

Article em En | MEDLINE | ID: mdl-35216669

Assuntos

Sistemas CRISPR-Cas; Endonucleases; Sistema Livre de Células/metabolismo; DNA/genética; Endonucleases/genética; RNA/metabolismo

Palavras-chave

CAST; Cascade; PAM; PAM wheel; PAM-DETECT; TXTL; Type I CRISPR-Cas system; high-throughput screen; restriction enzyme

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Endonucleases / Sistemas CRISPR-Cas Idioma: En Revista: Mol Cell Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha

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