Obtaining Reliable RT-qPCR Results in Molecular Diagnostics-MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery.

Grätz, Christian; Bui, Maria L U; Thaqi, Granit; Kirchner, Benedikt; Loewe, Robert P; Pfaffl, Michael W

Grätz, Christian; Bui, Maria L U; Thaqi, Granit; Kirchner, Benedikt; Loewe, Robert P; Pfaffl, Michael W.

Afiliação

Grätz C; Department of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany.
Bui MLU; GeneSurge GmbH, Ottostr. 3, 80333 München, Germany.
Thaqi G; Department of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany.
Kirchner B; GeneSurge GmbH, Ottostr. 3, 80333 München, Germany.
Loewe RP; Department of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany.
Pfaffl MW; Department of Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany.

Life (Basel) ; 12(3)2022 Mar 07.

Article em En | MEDLINE | ID: mdl-35330136

Palavras-chave

MIQE; RT-qPCR; biomarker signature; molecular diagnostics; transcriptional biomarkers

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Guideline Idioma: En Revista: Life (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha

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