Methanococcoides orientis sp. nov., a methylotrophic methanogen isolated from sediment of the East China Sea.
Int J Syst Evol Microbiol
; 72(5)2022 May.
Article
em En
| MEDLINE
| ID: mdl-35583432
ABSTRACT
A novel methylotrophic methanogen Methanococcoides orientis sp. nov. was isolated from East China Sea sediment. Type strain LMO-1T of Methanococcoides orientis sp. nov. was irregular 1-2 µm cocci without flagella. Strain LMO-1T could utilize a variety of methylated compounds including methanol, methylamine, dimethylamine and trimethylamine for growth and methanogenesis, while H2/CO2 or acetate could not be used for growth or methanogenesis. Optimum growth temperature was 30-35 °C, optimum pH range for growth was 7.0-7.5, while the optimum salinity spectrum for growth was 1.0%-5.0% NaCl. Based on 16S rRNA gene similarity, strain LMO-1T belongs to Methanococcoides, with the highest sequence similarity to Methanococcoides methylutens DSM 2657T (99.8â%), Methanococcoides vulcani SLH33T(99.4â%), followed by Methanococcoides alaskense AK-5T(98.1â%), Methanococcoides burtonii DSM 6242T (98.0â%). Digital DNA-DNA hybridization also showed highest similarity with Methanococcoides methylutens DSM 2657T, with the value of 58.4â%. The average nucleotide identity between strain LMO-1T and Methanococcoides methylutens DSM 2657T was 94.06â%. In summary, LMO-1T represents a novel species of the genus Methaococcoides, for which the name Methanococcoides orientis sp. nov. is proposed. The type strain is LMO-1T (=MCCC 4K00106T=JCM 39195T).
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Methanosarcinaceae
/
Ácidos Graxos
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article