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Quantifying Regulated Mitochondrial Fission in Macrophages.
Afroz, Syeda Farhana; Condon, Nicholas D; Sweet, Matthew J; Kapetanovic, Ronan.
Afiliação
  • Afroz SF; Institute for Molecular Bioscience (IMB), The University of Queensland, Brisbane, QLD, Australia.
  • Condon ND; IMB Centre for Inflammation and Disease Research, The University of Queensland, Brisbane, QLD, Australia.
  • Sweet MJ; Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia.
  • Kapetanovic R; Institute for Molecular Bioscience (IMB), The University of Queensland, Brisbane, QLD, Australia.
Methods Mol Biol ; 2523: 281-301, 2022.
Article em En | MEDLINE | ID: mdl-35759204
Mitochondria have co-evolved with eukaryotic cells for more than a billion years, becoming an important cog in their machinery. They are best known for being tasked with energy generation through the production of adenosine triphosphate, but they also have roles in several other cellular processes, for example, immune and inflammatory responses. Mitochondria have important functions in macrophages, key innate immune cells that detect pathogens and drive inflammation. Mitochondrial activity is influenced by the highly dynamic nature of the mitochondrial network, which alternates between interconnected tubular and fragmented forms. The dynamic balance between this interconnected fused network and fission-mediated mitochondrial fragmentation modulates inflammatory responses such as production of cytokines and mitochondrial reactive oxygen species. Here we describe methods to differentiate mouse bone marrow cells into macrophages and the use of light microscopy, electron microscopy, flow cytometry, and Western blotting to quantify regulated mitochondrial dynamics in these differentiated macrophages.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dinâmica Mitocondrial / Mitocôndrias Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dinâmica Mitocondrial / Mitocôndrias Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália