Identification of MPK4 kinase interactome using TurboID proximity labeling proteomics in Arabidopsis thaliana.
Methods Enzymol
; 676: 369-384, 2022.
Article
em En
| MEDLINE
| ID: mdl-36280358
ABSTRACT
TurboID is a new and efficient proximity labeling system that was first developed in living mammalian cells. TurboID is a modified bacterial biotin ligase that can be fused to a bait protein, which can then modify proximal interacting proteins with biotin. Prey proteins subsequently labeled with biotin tags will be pulled down with streptavidin-coated beads and identified by mass spectrometry-based proteomics. TurboID has been recently applied to living plant cells and provided promising results in identification of interacting proteins. Mitogen-activated protein kinase 4 (MPK4) is important for plant growth, development, and defense; however, the molecular mechanisms underlying the range of MPK4 functions are not completely known. Here we use modern proteomics together with the TurboID in a proof-of-concept study to profile the MPK4 interactome and uncover the functions of MPK4 in plant signaling cascades.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Proteínas de Arabidopsis
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos