Allelopathic effect of benzoic acid (hydroponics root exudate) on microalgae growth.

Hydroponic effluent (HE) contains a reasonable amount of residual nutrients. Therefore, HE could be used as a low-cost growth media for microalgae mediated resource recovery and water recycling. However, the presence of root exudates (particularly, benzoic acid) may lead to toxicity in microalgae.In the present study, the allelopathic effects of benzoic acid on microalgal growth was tested. During 96 h batch growth, Chlorella pyrenoidosa showed the highest biomass concentration (0.064-0.037 g.L-1) compared to Chlorella sorokiniana (0.09-0.26 g.L-1) at the tested benzoic acid doses. Moreover, both the species showed growth stimulation and growth inhibition up to certain benzoic acid doses. Hence, both the microalgal species showed allelopathic behaviour at different doses of benzoic acid. Further, the observed half effective concentration (96 h EC50) were 65.10 mg.L-1 and 105.27 mg.L-1, respectively, for Chlorella pyrenoidosa and C. sorokiniana with 95% confidence limits. Further, Haldane's model best fitted with experimental data of both the microalgae (r âˆ¼ 0.99). Overall, the study reveals that the HE with low benzoic acid dose may serve as a suitable growth media for microalgae. However, further in-depth research interventions using real HE are desirable to determine its real-world applicability.