Your browser doesn't support javascript.
loading
Live births from urine derived cells.
Olsson, P Olof; Yeonwoo, Jeong; Park, Kyumi; Yoo, Yeong-Min; Hwang, W S.
Afiliação
  • Olsson PO; Fujairah Genetics Center, Fujairah, UAE.
  • Yeonwoo J; UAE Biotech Research Center, Abu Dhabi, UAE.
  • Park K; Department of Companion Animal & Animal Resources Science, Joongbu University, Geumsan-gun, Republic of Korea.
  • Yoo YM; Lab of Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea.
  • Hwang WS; UAE Biotech Research Center, Abu Dhabi, UAE.
PLoS One ; 18(1): e0278607, 2023.
Article em En | MEDLINE | ID: mdl-36696395
ABSTRACT
Here we report urine-derived cell (UDC) culture and subsequent use for cloning which resulted in the successful development of cloned canine pups, which have remained healthy into adulthood. Bovine UDCs were used in vitro to establish comparative differences between cell sources. UDCs were chosen as a readily available and noninvasive source for obtaining cells. We analyzed the viability of cells stored in urine over time and could consistently culture cells which had remained in urine for 48hrs. Cells were shown to be viable and capable of being transfected with plasmids. Although primarily of epithelial origin, cells were found from multiple lineages, indicating that they enter the urine from more than one source. Held in urine, at 4°C, the majority of cells maintained their membrane integrity for several days. When compared to in vitro fertilization (IVF) derived embryos or those from traditional SCNT, UDC derived embryos did not differ in total cell number or in the number of DNA breaks, measured by TUNEL stain. These results indicate that viable cells can be obtained from multiple species' urine, capable of being used to produce live offspring at a comparable rate to other cell sources, evidenced by a 25% pregnancy rate and 2 live births with no losses in the canine UDC cloning trial. This represents a noninvasive means to recover the breeding capacity of genetically important or infertile animals. Obtaining cells in this way may provide source material for human and animal studies where cells are utilized.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clonagem de Organismos / Nascido Vivo Limite: Animals / Pregnancy Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clonagem de Organismos / Nascido Vivo Limite: Animals / Pregnancy Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2023 Tipo de documento: Article