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Anti-inflammatory potential of Penicillium brefeldianum endophytic fungus supported with phytochemical profiling.
Saleh, Asmaa; Negm, Walaa A; El-Masry, Thanaa A; Eliwa, Duaa; Alotaibi, Badriyah; Alosaimi, Manal E; Alotaibi, Khalid Nijr; Magdeldin, Sameh; Mahgoub, Sebaey; Elekhnawy, Engy.
Afiliação
  • Saleh A; Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia.
  • Negm WA; Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt. walaa.negm@pharm.tanta.edu.eg.
  • El-Masry TA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
  • Eliwa D; Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
  • Alotaibi B; Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia.
  • Alosaimi ME; Department of Basic Health Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia.
  • Alotaibi KN; Health Services Directorate, Ministry of Defense, Riyadh, 84428, Saudi Arabia.
  • Magdeldin S; Proteomics and Metabolomics Research Program, Department of Basic Research, Children's Cancer Hospital 57357, Cairo, 11441, Egypt.
  • Mahgoub S; Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
  • Elekhnawy E; Proteomics and Metabolomics Research Program, Department of Basic Research, Children's Cancer Hospital 57357, Cairo, 11441, Egypt.
Microb Cell Fact ; 22(1): 83, 2023 Apr 27.
Article em En | MEDLINE | ID: mdl-37106372
ABSTRACT
Various factors contribute to the development of the acute inflammation process, like the pro-inflammatory cytokines, certain enzymes as well as oxidative stress mediators. The anti-inflammatory potential of the endophytic fungus Penicillium brefeldianum was explored in carrageenan-induced inflammation in rats. After isolation of the fungus from Acalypha hispida leaves, it was identified by 18S rRNA gene sequencing. Then, its phytochemical profile was elucidated using LC-ESI-MS/MS technique. There was a remarkable decrease in the edema weight in the endophytic fungi-treated group (200 mg/kg). Also, this group had few inflammatory cells and thickened epidermis with underlying moderate collagenosis when stained with haematoxylin and eosin. Besides, immunostaining with monoclonal antibodies of cyclooxygenase-2 and tumor necrosis factor alpha showed a decrease in the positive immune cells in the endophytic fungi treated group (200 mg/kg) in relation to the positive control. Interestingly, the levels of the inflammatory as well as oxidative stress markers, including prostaglandin E2, nitric oxide, and malondialdehyde, which are hallmarks of the inflammatory process, considerably diminished (p < 0.05) in this group. qRT-PCR was utilised to elucidate the impact of the endophytic fungi treatment on the expression of interleukins (IL-1ß and IL-6) genes, which decreased in comparison with the positive control group. Consequently, we can deduce that P. brefeldianum endophytic fungus has a promising anti-inflammatory potential and should be extensively studied on a broader range in the near future.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicillium / Espectrometria de Massas em Tandem Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Arábia Saudita

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Penicillium / Espectrometria de Massas em Tandem Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Arábia Saudita