Specific base catalysis by yeast alcohol dehydrogenase I with substitutions of histidine-48 by glutamate or serine residues in the proton relay system.

Plapp, Bryce V; Kratzer, Darla Ann; Souhrada, Susan K; Warth, Edda; Jacobi, Tobias

Plapp, Bryce V; Kratzer, Darla Ann; Souhrada, Susan K; Warth, Edda; Jacobi, Tobias.

Afiliação

Plapp BV; Department of Biochemistry and Molecular Biology, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: bv-plapp@uiowa.edu.
Kratzer DA; Department of Biochemistry and Molecular Biology, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: pastorkratzer@gmail.com.
Souhrada SK; Department of Biochemistry and Molecular Biology, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: souhrada@iastate.edu.
Warth E; Department of Biochemistry and Molecular Biology, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: Edda.Warth@rpda.hessen.de.
Jacobi T; Department of Biochemistry and Molecular Biology, The University of Iowa, Iowa City, IA, 52242, USA. Electronic address: Tobias.Jacobi@mkuem.rlp.de.

Chem Biol Interact ; 382: 110558, 2023 Sep 01.

Article em En | MEDLINE | ID: mdl-37247811

Assuntos

Álcool Desidrogenase; Ácido Glutâmico; Animais; Cavalos; Álcool Desidrogenase/metabolismo; NAD/metabolismo; Prótons; Histidina; Deutério; Serina; Mutagênese Sítio-Dirigida; Saccharomyces cerevisiae/metabolismo; Catálise; Etanol; Acetaldeído; Cinética; Concentração de Íons de Hidrogênio

Palavras-chave

Enzyme kinetics; Enzyme mechanism; Hydroxide catalysis; Mutagenesis; Proton transfer

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Álcool Desidrogenase / Ácido Glutâmico Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2023 Tipo de documento: Article

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