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Blood-borne extracellular vesicles of bacteria and intestinal cells in patients with psychotic disorders.
Tunset, Mette Elise; Haslene-Hox, Hanne; Van Den Bossche, Tim; Maleki, Susan; Vaaler, Arne; Kondziella, Daniel.
Afiliação
  • Tunset ME; Department of Psychosis and Rehabilitation, Psychiatry Clinic, St. Olavs University Hospital, Trondheim, Norway.
  • Haslene-Hox H; Department of Mental Health, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
  • Van Den Bossche T; Department of Biotechnology and Nanomedicine, SINTEF, Trondheim, Norway.
  • Maleki S; VIB - UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
  • Vaaler A; Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
  • Kondziella D; Department of Biotechnology and Nanomedicine, SINTEF, Trondheim, Norway.
Nord J Psychiatry ; 77(7): 686-695, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37354486
BACKGROUND: Human cells and bacteria secrete extracellular vesicles (EV) which play a role in intercellular communication. EV from the host intestinal epithelium are involved in the regulation of bacterial gene expression and growth. Bacterial EV (bactEV) produced in the intestine can pass to various tissues where they deliver biomolecules to many kinds of cells, including neurons. Emerging data indicate that gut microbiota is altered in patients with psychotic disorders. We hypothesized that the amount and content of blood-borne EV from intestinal cells and bactEV in psychotic patients would differ from healthy controls. METHODS: We analyzed for human intestinal proteins by proteomics, for bactEV by metaproteomic analysis, and by measuring the level of lipopolysaccharide (LPS) in blood-borne EV from patients with psychotic disorders (n = 25), tested twice, in the acute phase of psychosis and after improvement, with age- and sex-matched healthy controls (n = 25). RESULTS: Patients with psychotic disorders had lower LPS levels in their EV compared to healthy controls (p = .027). Metaproteome analyses confirmed LPS finding and identified Firmicutes and Bacteroidetes as dominating phyla. Total amounts of human intestine proteins in EV isolated from blood was lower in patients compared to controls (p = .02). CONCLUSIONS: Our results suggest that bactEV and host intestinal EV are decreased in patients with psychosis and that this topic is worthy of further investigation given potential pathophysiological implications. Possible mechanisms involve dysregulation of the gut microbiota by host EV, altered translocation of bactEV to systemic circulation where bactEV can interact with both the brain and the immune system.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtornos Psicóticos / Vesículas Extracelulares Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nord J Psychiatry Assunto da revista: PSICOFARMACOLOGIA / PSIQUIATRIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Noruega

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtornos Psicóticos / Vesículas Extracelulares Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nord J Psychiatry Assunto da revista: PSICOFARMACOLOGIA / PSIQUIATRIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Noruega