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Comparative in vivo biodistribution of cells labelled with [89Zr]Zr-(oxinate)4 or [89Zr]Zr-DFO-NCS using PET.
Friberger, Ida; Nilsson, Joachim N; Lu, Li; Siikanen, Jonathan; Ardenfors, Oscar; Milton, Stefan; Samén, Erik; Goos, Jeroen A C M; Carlsten, Mattias; Holmin, Staffan; Tran, Thuy A.
Afiliação
  • Friberger I; Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden. ida.friberger@ki.se.
  • Nilsson JN; Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden.
  • Lu L; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Siikanen J; Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Ardenfors O; Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden.
  • Milton S; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden.
  • Samén E; Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden.
  • Goos JACM; Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Carlsten M; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden.
  • Holmin S; Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden.
  • Tran TA; Department of Radiopharmacy, Karolinska University Hospital, Stockholm, Sweden.
EJNMMI Res ; 13(1): 73, 2023 Aug 08.
Article em En | MEDLINE | ID: mdl-37552341
BACKGROUND: In vivo monitoring of cell biodistribution using positron emission tomography (PET) provides a quantitative non-invasive method to further optimize cell therapies and related new developments in the field. Our group has earlier optimized and evaluated the in vitro properties of two radiotracers,[89Zr]Zr-(oxinate)4 and [89Zr]Zr-DFO-NCS, for the radiolabelling of different cell types. Here, we performed a microPET study to assess the in vivo biodistribution of cells in rats using these two radiotracers. Human decidual stromal cells (hDSC) and rat macrophages (rMac) were radiolabelled with [89Zr]Zr-(oxinate)4 or [89Zr]Zr-DFO-NCS. Rats were intravenously injected with radiolabelled cells, and the in vivo biodistribution was monitored with microPET/CT imaging for up to day 7. Organ uptake was evaluated and presented as a percentage of injected activity per gram tissue (%IA/g) and total absorbed organ doses (mSv/MBq). RESULTS: The biodistribution in vivo showed an immediate uptake in the lungs. Thereafter, [89Zr]Zr-(oxinate)4 labelled cells migrated to the liver, while the signal from [89Zr]Zr-DFO-NCS labelled cells lingered in the lungs. The differences in the in vivo behaviour for the same cell type appeared related to the radiotracer labelling. After 24 h, [89Zr]Zr-(oxinate)4 labelled cells had over 70% higher liver uptake for both hDSC and rMac compared to [89Zr]Zr-DFO-NCS labelled cells, whereas [89Zr]Zr-DFO-NCS labelled cells showed over 60% higher uptake in the lungs compared to [89Zr]Zr-(oxinate)4 labelled cells. This difference in both lung and liver uptake continued until day 7. Dosimetry calculations showed a higher effective dose (mSv/MBq) for [89Zr]Zr-DFO-NCS compared to [89Zr]Zr-(oxinate)4, for both cell types. Although the bone uptake was higher for [89Zr]Zr-(oxinate)4 labelled cells, the prolonged uptake in the lungs contributed to a significant crossfire to bone marrow resulting in a higher bone dose. CONCLUSION: The [89Zr]Zr-DFO-NCS labelled cells suggest a prolonged accumulation in the lungs, while [89Zr]Zr-(oxinate)4 suggests quicker clearance of the lungs followed by accumulation in the liver. Accumulation of radiolabelled cells in the liver corresponds to other cell-tracking methods. Further studies are required to determine the actual location of the [89Zr]Zr-DFO-NCS labelled cell.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: EJNMMI Res Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Suécia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: EJNMMI Res Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Suécia