Combining E-ice-COLD-PCR and Pyrosequencing with Di-Base Addition (PDBA) Enables Sensitive Detection of Low-Abundance Mutations.

Pu, Dan; Chen, Huimin; Fu, Wenjuan; Cui, Youhong; Shu, Kunxian

Pu, Dan; Chen, Huimin; Fu, Wenjuan; Cui, Youhong; Shu, Kunxian.

Afiliação

Pu D; Chongqing Key Laboratory of Big Data for Bio Intelligence, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China.
Chen H; Chongqing Key Laboratory of Big Data for Bio Intelligence, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China.
Fu W; Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
Cui Y; Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China. cuiyouhongx@163.com.
Shu K; Chongqing Key Laboratory of Big Data for Bio Intelligence, Chongqing University of Posts and Telecommunications, Chongqing, 400065, China. shukx@cqupt.edu.cn.

Appl Biochem Biotechnol ; 2023 Oct 21.

Article em En | MEDLINE | ID: mdl-37864708

Palavras-chave

BRAF V600E mutation; Di-base; E-ice-COLD-PCR; Low-abundance mutations; Pyrosequencing

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

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