Protocatechuate hydroxylase is a novel group A flavoprotein monooxygenase with a unique substrate recognition mechanism.

Katsuki, Nozomi; Fukushima, Riku; Doi, Yuki; Masuo, Shunsuke; Arakawa, Takatoshi; Yamada, Chihaya; Fushinobu, Shinya; Takaya, Naoki

Katsuki, Nozomi; Fukushima, Riku; Doi, Yuki; Masuo, Shunsuke; Arakawa, Takatoshi; Yamada, Chihaya; Fushinobu, Shinya; Takaya, Naoki.

Afiliação

Katsuki N; Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Fukushima R; Department of Biotechnology, The University of Tokyo, Tokyo, Japan.
Doi Y; Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Masuo S; Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Arakawa T; Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
Yamada C; School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
Fushinobu S; Department of Biotechnology, The University of Tokyo, Tokyo, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan. Electronic address: asfushi@mail.ecc.u-tokyo.ac.jp.
Takaya N; Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability, University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address: takaya.naoki.ge@u.tsukuba.ac.jp.

J Biol Chem ; 300(1): 105508, 2024 Jan.

Article em En | MEDLINE | ID: mdl-38029967

Assuntos

4-Hidroxibenzoato-3-Mono-Oxigenase; Pseudomonas; 4-Hidroxibenzoato-3-Mono-Oxigenase/metabolismo; Sítios de Ligação; Flavoproteínas/genética; Flavoproteínas/metabolismo; Cinética; Oxigenases de Função Mista/genética; Oxigenases de Função Mista/metabolismo; Filogenia; Pseudomonas/enzimologia; Pseudomonas/metabolismo; Xylophilus/enzimologia

Palavras-chave

crystal structure; enzyme kinetics; flavin adenine dinucleotide; p-hydroxybenzoate hydroxylase; pre-steady state kinetics; pyridine nucleotide; reductive half-reaction

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas / 4-Hidroxibenzoato-3-Mono-Oxigenase Idioma: En Revista: J Biol Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão

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