Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins.
Anaerobe
; 85: 102817, 2024 Feb.
Article
em En
| MEDLINE
| ID: mdl-38163631
ABSTRACT
OBJECTIVES:
This study aimed to produce and purify Clostridium perfringens type C beta-toxin, sheep anti-beta toxin immunoglobulin G (IgG) and chicken immunoglobulin Y (IgY).METHODS:
Two methods were used for beta-toxin purification single-step metal affinity chromatography (MAC) using zinc as a chelator and ion exchange chromatography (IEX). The purified and inactivated beta-toxoids were then administered to sheep and chickens in order to produce IgG and IgY.RESULTS:
All assays using the IEX failed. In contrast, MAC purified more than 21 mg of toxin per run in a single-step protocol. The purified and inactivated beta-toxoids were then administered to sheep and chickens, and IgG and IgY were purified with a high yield, medium antibody titer of 50 IU/mL, and high avidity (73.2 %).CONCLUSIONS:
C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Imunoglobulinas
/
Antitoxinas
/
Infecções por Clostridium
/
Enterite
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Anaerobe
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Brasil