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MiR-17-5p-engineered sEVs Encapsulated in GelMA Hydrogel Facilitated Diabetic Wound Healing by Targeting PTEN and p21.
Wei, Qian; Su, Jianlong; Meng, Sheng; Wang, Yaxi; Ma, Kui; Li, Bingmin; Chu, Ziqiang; Huang, Qilin; Hu, Wenzhi; Wang, Zihao; Tian, Lige; Liu, Xi; Li, Tanshi; Fu, Xiaobing; Zhang, Cuiping.
Afiliação
  • Wei Q; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Su J; Research Unit of Trauma Care, Tissue Repair and Regeneration, Chinese Academy of Medical Sciences, 2019RU051, Beijing, 100048, P. R. China.
  • Meng S; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Wang Y; Chinese PLA Medical School, Beijing, 100853, P. R. China.
  • Ma K; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Li B; Chinese PLA Medical School, Beijing, 100853, P. R. China.
  • Chu Z; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Huang Q; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Hu W; Research Unit of Trauma Care, Tissue Repair and Regeneration, Chinese Academy of Medical Sciences, 2019RU051, Beijing, 100048, P. R. China.
  • Wang Z; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Tian L; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Liu X; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Li T; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Fu X; Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division, Chinese PLA General Hospital, Beijing, 100048, P. R. China.
  • Zhang C; Chinese PLA Medical School, Beijing, 100853, P. R. China.
Adv Sci (Weinh) ; 11(13): e2307761, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38286650
ABSTRACT
Delayed wound healing is a major complication of diabetes, and is associated with impaired cellular functions. Current treatments are unsatisfactory. Based on the previous reports on microRNA expression in small extracellular vesicles (sEVs), miR-17-5p-engineered sEVs (sEVs17-OE) and encapsulated them in gelatin methacryloyl (GelMA) hydrogel for diabetic wounds treatment are fabricated. SEVs17-OE are successfully fabricated with a 16-fold increase in miR-17-5p expression. SEVs17-OE inhibited senescence and promoted the proliferation, migration, and tube formation of high glucose-induced human umbilical vein endothelial cells (HG-HUVECs). Additionally, sEVs17-OE also performs a promotive effect on high glucose-induced human dermal fibroblasts (HG-HDFs). Mechanism analysis showed the expressions of p21 and phosphatase and tensin homolog (PTEN), as the target genes of miR-17-5p, are downregulated significantly by sEVs17-OE. Accordingly, the downstream genes and pathways of p21 and PTEN, are activated. Next, sEVs17-OE are loaded in GelMA hydrogel to fabricate a novel bioactive wound dressing and to evaluate their effects on diabetic wound healing. Gel-sEVs17-OE effectively accelerated wound healing by promoting angiogenesis and collagen deposition. The cellular mechanism may be associated with local cell proliferation. Therefore, a novel bioactive wound dressing by loading sEVs17-OE in GelMA hydrogel, offering an option for chronic wound management is successfully fabricated.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / MicroRNAs / Diabetes Mellitus / Vesículas Extracelulares / Gelatina / Metacrilatos Limite: Humans Idioma: En Revista: Adv Sci (Weinh) Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / MicroRNAs / Diabetes Mellitus / Vesículas Extracelulares / Gelatina / Metacrilatos Limite: Humans Idioma: En Revista: Adv Sci (Weinh) Ano de publicação: 2024 Tipo de documento: Article