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Mapping microhabitats of lignocellulose decomposition by a microbial consortium.
Velickovic, Marija; Wu, Ruonan; Gao, Yuqian; Thairu, Margaret W; Velickovic, Dusan; Munoz, Nathalie; Clendinen, Chaevien S; Bilbao, Aivett; Chu, Rosalie K; Lalli, Priscila M; Zemaitis, Kevin; Nicora, Carrie D; Kyle, Jennifer E; Orton, Daniel; Williams, Sarai; Zhu, Ying; Zhao, Rui; Monroe, Matthew E; Moore, Ronald J; Webb-Robertson, Bobbie-Jo M; Bramer, Lisa M; Currie, Cameron R; Piehowski, Paul D; Burnum-Johnson, Kristin E.
Afiliação
  • Velickovic M; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Wu R; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Gao Y; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Thairu MW; Wisconsin Institute for Discovery and Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, USA.
  • Velickovic D; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Munoz N; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Clendinen CS; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Bilbao A; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Chu RK; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Lalli PM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Zemaitis K; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Nicora CD; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Kyle JE; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Orton D; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Williams S; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Zhu Y; Department of Microchemistry, Proteomics, Lipidomics, and Next Generation Sequencing, Genentech, San Francisco, CA, USA.
  • Zhao R; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Monroe ME; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Moore RJ; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Webb-Robertson BM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Bramer LM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Currie CR; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.
  • Piehowski PD; Department of Biochemistry & Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
  • Burnum-Johnson KE; The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
Nat Chem Biol ; 2024 Feb 01.
Article em En | MEDLINE | ID: mdl-38302607
ABSTRACT
The leaf-cutter ant fungal garden ecosystem is a naturally evolved model system for efficient plant biomass degradation. Degradation processes mediated by the symbiotic fungus Leucoagaricus gongylophorus are difficult to characterize due to dynamic metabolisms and spatial complexity of the system. Herein, we performed microscale imaging across 12-µm-thick adjacent sections of Atta cephalotes fungal gardens and applied a metabolome-informed proteome imaging approach to map lignin degradation. This approach combines two spatial multiomics mass spectrometry modalities that enabled us to visualize colocalized metabolites and proteins across and through the fungal garden. Spatially profiled metabolites revealed an accumulation of lignin-related products, outlining morphologically unique lignin microhabitats. Metaproteomic analyses of these microhabitats revealed carbohydrate-degrading enzymes, indicating a prominent fungal role in lignocellulose decomposition. Integration of metabolome-informed proteome imaging data provides a comprehensive view of underlying biological pathways to inform our understanding of metabolic fungal pathways in plant matter degradation within the micrometer-scale environment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos