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Increased level of calcitonin mRNA after 1,25-dihydroxyvitamin D3 injection in the rat.
FEBS Lett ; 184(2): 268-72, 1985 May 20.
Article em En | MEDLINE | ID: mdl-3838942
Vitamin D metabolites are able to change plasma calcitonin (CT) levels, but nothing is known about a possible effect at the CT gene level. Here we have investigated the acute effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on the CT biosynthetic activity of thyroid glands from adult rats. Plasma CT levels were significantly increased (X2) 1 and 2 h after 1,25-(OH)2D3 injection in the face of unchanged plasma calcium values. The thyroidal CT content also was unchanged. A 2-fold increase in CT mRNA level measured by dot-blot hybridization occurred 1 and 2 h after 1,25-(OH)2D3 administration. Expression of CT gene products was examined in the rabbit reticulocyte lysate cell-free translation assay. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographed and quantified by integration. A single precursor of Mr approximately equal to 15 000 could be specifically immunoprecipitated with CT antisera. A 3-4-fold rise in translatable CT mRNA activity was observed 1 and 2 h after 1,25-(OH)2D3 injection. Thus, parallel changes in CT mRNA level, CT mRNA activity and plasma CT levels were observed in adult female rats after administration of 1,25-(OH)2D3. These findings demonstrate for the first time that 1,25-(OH)2D3 enhanced CT gene expression in the face of unchanged plasma calcium levels.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcitriol / Calcitonina Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 1985 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcitriol / Calcitonina Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 1985 Tipo de documento: Article