Antibodies from chlamydia-infected individuals facilitate phagocytosis via Fc receptors.
Infect Immun
; 92(4): e0050323, 2024 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-38451079
ABSTRACT
Non-neutralizing functions of antibodies, including phagocytosis, may play a role in Chlamydia trachomatis (CT) infection, but these functions have not been studied and assays are lacking. We utilized a flow-cytometry-based assay to determine whether serum samples from a well-characterized cohort of CT-infected and naïve control individuals enhanced phagocytosis via Fc-receptor-expressing THP-1 cells, and whether this activity correlated with antibody titers. Fc-receptor-mediated phagocytosis was detected only in CT+ donors. Phagocytosis generally did not correlate well with antibody titer. In addition, we found that complement from both CT+ and negative individuals enhanced phagocytosis of CT into primary neutrophils. These results suggest that anti-CT antibodies can have functions that are not reflected by titer. This method could be used to quantitively measure Fc-receptor-mediated function of anti-CT antibodies or complement activity and could reveal new immune correlates of protection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Fc
/
Infecções por Chlamydia
Limite:
Humans
Idioma:
En
Revista:
Infect Immun
/
Infect. immun. (Online)
/
Infection and immunity (Online)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos