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One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16.
Magkouta, Sophia; Veroutis, Dimitris; Pousias, Athanasios; Papaspyropoulos, Angelos; Giannetti, Kety; Pippa, Natassa; Lougiakis, Nikolaos; Kambas, Konstantinos; Lagopati, Nefeli; Polyzou, Aikaterini; Georgiou, Maria; Chountoulesi, Maria; Pispas, Stergios; Foutadakis, Spyros; Kyrodimos, Efthymios; Pouli, Nicole; Marakos, Panagiotis; Kotsinas, Athanassios; Verginis, Panayotis; Valakos, Dimitrios; Vatsellas, Giannis; Petty, Russell; Thanos, Dimitris; Demaria, Marco; Evangelou, Konstantinos; Di Micco, Raffaella; Gorgoulis, Vassilis G.
Afiliação
  • Magkouta S; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Marianthi Simou and G.P. Livanos Labs, 1st Department of Critical Care and Pulmonary Services, School of Medicine, National & Kapodistrian
  • Veroutis D; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Pousias A; Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
  • Papaspyropoulos A; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Giannetti K; San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.
  • Pippa N; Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimioupolis Zografou, 15771 Athens, Greece; Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 11635 Athens, Greece.
  • Lougiakis N; Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
  • Kambas K; Hellenic Pasteur Institute, 11521 Athens, Greece.
  • Lagopati N; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece; Laboratory of Biology, Department of Basic Medical Sciences, Medical
  • Polyzou A; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
  • Georgiou M; Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
  • Chountoulesi M; Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimioupolis Zografou, 15771 Athens, Greece.
  • Pispas S; Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 11635 Athens, Greece.
  • Foutadakis S; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Kyrodimos E; First ENT Department, Hippocration Hospital, National Kapodistrian University of Athens, 11527 Athens, GR, Greece.
  • Pouli N; Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
  • Marakos P; Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15771 Athens, Greece.
  • Kotsinas A; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
  • Verginis P; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece; Laboratory of Immune Regulation and Tolerance, Division of Basic Sciences, University of Crete Medical School, 70013 Heraklion, Greece; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, 7
  • Valakos D; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Vatsellas G; Greek Genome Center, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Petty R; Ninewells Hospital and Medical School, University of Dundee, Dundee DD19SY, UK.
  • Thanos D; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece; Greek Genome Center, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
  • Demaria M; European Research Institute for the Biology of Ageing (ERIBA), University Medical Center Groningen, Groningen 9713 AV, The Netherlands.
  • Evangelou K; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece.
  • Di Micco R; San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.
  • Gorgoulis VG; Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece; Ninewells Hospital and Medical School, University of Dundee, Dundee D
STAR Protoc ; 5(1): 102929, 2024 Mar 15.
Article em En | MEDLINE | ID: mdl-38460134
ABSTRACT
Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senescência Celular / Corantes Fluorescentes Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senescência Celular / Corantes Fluorescentes Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article