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Early Increase in Blood-Brain Barrier Permeability in a Murine Model Exposed to Fifteen Days of Intermittent Hypoxia.
Roche, Frederic; Briançon-Marjollet, Anne; Dematteis, Maurice; Baldazza, Marie; Gonthier, Brigitte; Bertholon, Frederique; Perek, Nathalie; Pépin, Jean-Louis.
Afiliação
  • Roche F; INSERM, SAINBIOSE U1059, Université Jean Monnet Saint-Étienne, Mines Saint Etienne, F-42023 Saint-Étienne, France.
  • Briançon-Marjollet A; INSERM U1300, HP2 Laboratory, Université Grenoble Alpes, F-38042 Grenoble, France.
  • Dematteis M; INSERM U1300, HP2 Laboratory, Université Grenoble Alpes, F-38042 Grenoble, France.
  • Baldazza M; INSERM U1300, HP2 Laboratory, Université Grenoble Alpes, F-38042 Grenoble, France.
  • Gonthier B; INSERM U1300, HP2 Laboratory, Université Grenoble Alpes, F-38042 Grenoble, France.
  • Bertholon F; INSERM U1300, HP2 Laboratory, Université Grenoble Alpes, F-38042 Grenoble, France.
  • Perek N; Centre de Ressources Biologiques, CHU de Saint Etienne, F-42055 Saint-Étienne, France.
  • Pépin JL; INSERM, SAINBIOSE U1059, Université Jean Monnet Saint-Étienne, Mines Saint Etienne, F-42023 Saint-Étienne, France.
Int J Mol Sci ; 25(5)2024 Mar 06.
Article em En | MEDLINE | ID: mdl-38474310
ABSTRACT
Obstructive sleep apnea (OSA) is characterized by intermittent repeated episodes of hypoxia-reoxygenation. OSA is associated with cerebrovascular consequences. An enhanced blood-brain barrier (BBB) permeability has been proposed as a marker of those disorders. We studied in mice the effects of 1 day and 15 days intermittent hypoxia (IH) exposure on BBB function. We focused on the dorsal part of the hippocampus and attempted to identify the molecular mechanisms by combining in vivo BBB permeability (Evans blue tests) and mRNA expression of several junction proteins (zona occludens (ZO-1,2,3), VE-cadherin, claudins (1,5,12), cingulin) and of aquaporins (1,4,9) on hippocampal brain tissues. After 15 days of IH exposure we observed an increase in BBB permeability, associated with increased mRNA expressions of claudins 1 and 12, aquaporins 1 and 9. IH seemed to increase early for claudin-1 mRNA expression as it doubled with 1 day of exposure and returned near to its base level after 15 days. Claudin-1 overexpression may represent an immediate response to IH exposure. Then, after 15 days of exposure, an increase in functional BBB permeability was associated with enhanced expression of aquaporin. These BBB alterations are possibly associated with a vasogenic oedema that may affect brain functions and accelerate neurodegenerative processes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aquaporinas / Apneia Obstrutiva do Sono Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aquaporinas / Apneia Obstrutiva do Sono Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França