Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum).
Arch Virol
; 169(5): 90, 2024 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-38578314
ABSTRACT
Trees and shrubs provide important ecological services. However, few studies have surveyed the virome in trees and shrubs. In this study, we discovered a new positive-sense RNA virus originating from Viburnum odoratissimum, which we named "Vo narna-like virus". The complete genome of Vo narna-like virus is 3,451 nt in length with an open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRP) protein. Phylogenetic analysis placed this virus within the betanarnavirus clade, sharing 53.63% amino acid sequence identity with its closest relative, Qingdao RNA virus 2. The complete sequence of the virus was confirmed by rapid amplification of cDNA ends (RACE) and Sanger sequencing. Small interfering RNA (siRNA) analysis indicated that this virus interacts with the RNA interference (RNAi) pathway of V. odoratissimum. This is the first report of a narnavirus in V. odoratissimum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
/
Viburnum
Idioma:
En
Revista:
Arch Virol
/
Arch. virol
/
Archives of virology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China