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NKS1/ELMO4 is an integral protein of a pectin synthesis protein complex and maintains Golgi morphology and cell adhesion in Arabidopsis.
Lathe, Rahul S; McFarlane, Heather E; Kesten, Christopher; Wang, Liu; Khan, Ghazanfar Abbas; Ebert, Berit; Ramírez-Rodríguez, Eduardo Antonio; Zheng, Shuai; Noord, Niels; Frandsen, Kristian; Bhalerao, Rishikesh P; Persson, Staffan.
Afiliação
  • Lathe RS; Copenhagen Plant Science Center, Department of Plant & Environmental Sciences, University of Copenhagen, Frederiksberg C 1871, Denmark.
  • McFarlane HE; Max-Planck Institute for Molecular Plant Physiology, Potsdam 14476, Germany.
  • Kesten C; Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå SE-90187, Sweden.
  • Wang L; Department of Cell & Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada.
  • Khan GA; School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia.
  • Ebert B; Copenhagen Plant Science Center, Department of Plant & Environmental Sciences, University of Copenhagen, Frederiksberg C 1871, Denmark.
  • Ramírez-Rodríguez EA; Copenhagen Plant Science Center, Department of Plant & Environmental Sciences, University of Copenhagen, Frederiksberg C 1871, Denmark.
  • Zheng S; School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia.
  • Noord N; School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia.
  • Frandsen K; Department of Animal, Plant and Soil Sciences, School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, VIC 3086, Australia.
  • Bhalerao RP; School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia.
  • Persson S; Department of Biology and Biotechnology, Ruhr University Bochum, Bochum 44780, Germany.
Proc Natl Acad Sci U S A ; 121(15): e2321759121, 2024 Apr 09.
Article em En | MEDLINE | ID: mdl-38579009
ABSTRACT
Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence critical agricultural characteristics, including fruit ripening and organ abscission. Middle lamellae are enriched in pectin polysaccharides, specifically homogalacturonan (HG). Here, we identify a plant-specific Arabidopsis DUF1068 protein, called NKS1/ELMO4, that is required for middle lamellae integrity and cell adhesion. NKS1 localizes to the Golgi apparatus and loss of NKS1 results in changes to Golgi structure and function. The nks1 mutants also display HG deficient phenotypes, including reduced seedling growth, changes to cell wall composition, and tissue integrity defects. These phenotypes are comparable to qua1 and qua2 mutants, which are defective in HG biosynthesis. Notably, genetic interactions indicate that NKS1 and the QUAs work in a common pathway. Protein interaction analyses and modeling corroborate that they work together in a stable protein complex with other pectin-related proteins. We propose that NKS1 is an integral part of a large pectin synthesis protein complex and that proper function of this complex is important to support Golgi structure and function.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Dinamarca

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Dinamarca