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Probiotic potential of a novel endophytic Streptomyces griseorubens CIBA-NS1 isolated from Salicornia sp. against Vibrio campbellii infection in shrimp.
Sudheer, N S; Biju, I F; Balasubramanian, C P; Panigrahi, A; Kumar, T Sathish; Kumar, Sujeet; Mandal, Babita; Das, S; De, D.
Afiliação
  • Sudheer NS; Kakdwip Research Centre of ICAR-CIBA, Kakdwip, South 24 Parganas, West Bengal, India. Electronic address: sudheer.s@icar.gov.in.
  • Biju IF; Kakdwip Research Centre of ICAR-CIBA, Kakdwip, South 24 Parganas, West Bengal, India.
  • Balasubramanian CP; ICAR-Central Institute of Brackishwater Aquaculture, Chennai, Tamil Nadu, India.
  • Panigrahi A; ICAR-Central Institute of Brackishwater Aquaculture, Chennai, Tamil Nadu, India.
  • Kumar TS; ICAR-Central Institute of Brackishwater Aquaculture, Chennai, Tamil Nadu, India.
  • Kumar S; ICAR-Central Institute of Brackishwater Aquaculture, Chennai, Tamil Nadu, India.
  • Mandal B; Kakdwip Research Centre of ICAR-CIBA, Kakdwip, South 24 Parganas, West Bengal, India.
  • Das S; Kakdwip Research Centre of ICAR-CIBA, Kakdwip, South 24 Parganas, West Bengal, India.
  • De D; Kakdwip Research Centre of ICAR-CIBA, Kakdwip, South 24 Parganas, West Bengal, India.
Microb Pathog ; 191: 106677, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38705217
ABSTRACT
A novel endophytic Streptomyces griseorubens CIBA-NS1 was isolated from a salt marsh plant Salicornia sp. The antagonistic effect of S. griseorubens against Vibrio campbellii, was studied both in vitro and in vivo. The strain was validated for its endophytic nature and characterized through scanning electron microscopy, morphological and biochemical studies and 16SrDNA sequencing. The salinity tolerance experiment has shown that highest antibacterial activity was at 40‰ (16 ± 1.4 mm) and lowest was at 10 ‰ salinity (6.94 ± 0.51 mm). In vivo exclusion of Vibrio by S. griseorubens CIBA-NS1 was studied in Penaeus indicus post larvae and evaluated for its ability to improve growth and survival of P. indicus. After 20 days administration of S. griseorubens CIBA-NS1, shrimps were challenged with V. campbellii. The S. griseorubens CIBA-NS1 reduced Vibrio population in test group when compared to control, improved survival (60.5 ± 6.4%) and growth, as indicated by weight gain (1.8 ± 0.05g). In control group survival and growth were 48.4 ± 3.5% and 1.4 ± 0.03 g respectively. On challenge with V. campbellii, the S. griseorubens CIBA-NS1 administered group showed better survival (85.6 ± 10%) than positive control (64.3 ± 10%). The results suggested that S. griseorubens CIBA-NS1 is antagonistic to V. campbellii, reduce Vibrio population in the culture system and improve growth and survival. This is the first report on antagonistic activity of S. griseorubens isolated from salt marsh plant Salicornia sp, as a probiotic candidate to prevent V. campbellii infection in shrimps.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Vibrio / Chenopodiaceae / Probióticos / Endófitos Limite: Animals Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Vibrio / Chenopodiaceae / Probióticos / Endófitos Limite: Animals Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article