Clostridium aquiflavi sp. nov., isolated from yellow water of Nongxiangxing baijiu in the Yibin region of China.
Int J Syst Evol Microbiol
; 74(6)2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38864839
ABSTRACT
A Gram-stain-positive, strictly anaerobic, endospore-forming and rod-shaped (0.6-0.8×2.7-13.1 µm) bacterium, designated as 5 N-1T, was isolated from a yellow water sample collected from the manufacturing process of Nongxiangxing baijiu in the Yibin region of Sichuan, PR China. Growth occurred at 15-40â°C (optimum growth at 37â°C), at pH 6.0-9.0 (optimum growth at pH 7.0) and in NaCl concentrations of 0-1â% (w/v) and ethanol concentrations of 0-2â% (v/v). The major fatty acids in strain 5 N-1T were C16ââ0, C18ââ0 and C14ââ0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and one unidentified lipid. Phylogenetic analysis of its 16S rRNA gene sequence indicated that strain 5 N-1T was most closely related to Clostridium weizhouense YB-6T (97.70â%) and Clostridium uliginosum DSM 12992T (97.56â%). The average nucleotide identity and digital DNAâDNA hybridization values between strain 5 N-1T and the above two type strains were 80.89 and 80.05â% and 25.80 and 25.30â%, respectively, which were all below the species thresholds. The genome size of strain 5 N-1T was 3.5 Mbp and the DNA G+C content was 27.5 mol%. Based on the results of phenotypic and genotypic analyses, strain 5 N-1T represents a novel species of the genus Clostridium, for which the name Clostridium aquiflavi sp. nov. is proposed. The type strain is Clostridium aquiflavi 5 N-1T (=CICC 24886T=JCM 35355T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Clostridium
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Análise de Sequência de DNA
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Ácidos Graxos
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Hibridização de Ácido Nucleico
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article