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Staphylococcus pseudintermedius and Pseudomonas aeruginosa Lubbock Chronic Wound Biofilm (LCWB): a suitable dual-species model for in vitro studies.
Di Lodovico, Silvia; Petrini, Morena; Di Fermo, Paola; De Pasquale, Valeria; De Martino, Luisa; D'Ercole, Simonetta; Nocera, Francesca Paola; Di Giulio, Mara.
Afiliação
  • Di Lodovico S; Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
  • Petrini M; Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" of Chieti, Italy.
  • Di Fermo P; Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" of Chieti, Italy.
  • De Pasquale V; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
  • De Martino L; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
  • D'Ercole S; Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" of Chieti, Italy.
  • Nocera FP; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy. Electronic address: francescapaola.nocera@unina.it.
  • Di Giulio M; Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
Microbes Infect ; : 105384, 2024 Jun 27.
Article em En | MEDLINE | ID: mdl-38944110
ABSTRACT
Antimicrobial treatment of methicillin-resistant Staphylococcus pseudintermedius associated with canine wounds represents an important challenge. The aim of this study was to create a canine wound infection model, Lubbock Chronic Wound Biofilm (LCWB), with a focus on S. pseudintermedius, drawing inspiration from the established human model involving Staphylococcus aureus. Methicillin-resistant S. pseudintermedius 115 (MRSP) and Pseudomonas aeruginosa 700 strains, isolated from dog wounds, were used to set up the LCWB at 24, 48 and 72 h. The LCWBs were evaluated in terms of volume, weight, and microbial CFU/mg. The microbial spatial distribution in the LCWBs was assessed by SEM and CLSM imaging. The best incubation time for the LCWB production in terms of volume (3.38 cm3 ± 0.13), weight (0.86 gr ± 0.02) and CFU/mg (up to 7.05 × 106 CFU/mg ± 2.89 × 105) was 48 h. The SEM and CLSM images showed a major viable microbial colonization at 48 h with non-mixed bacteria with a prevalence of MRSP on the surface and P. aeruginosa 700 in the depth of the wound. The obtained findings demonstrate the capability of S. pseudintermedius to grow together P. aeruginosa in the LCWB model, representing the suitable model to reproduce the animal chronic wound in vitro.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microbes Infect Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microbes Infect Assunto da revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália