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[Detection of Bacillus cereus-toxins (author's transl)]. / Nachweis von Bacillus cereus-Toxinen.
Zentralbl Bakteriol Orig A ; 238(2): 255-62, 1977 Jun.
Article em De | MEDLINE | ID: mdl-407743
During the logarithmic state of growth, B. cereus produced extracellular toxins, which could be precipitated by ammoniumsulphate. The toxins were not dialysable. 9 strains of B. cereus were tested and showed lethal, hemolytic, and phospholipase-C-reactions. The lethal toxin injected i.v., killed mice within 20 minutes. An intradermal injection of the 9 toxin-precipitates in rabbits produced skin reactions, which could be neutralized by a specific antiserum against the B. cereus strain B-4ac. Relatively high doses (50 mg/ml) of the toxin caused fluid accumulation after injection in ligated loops of rabbit gut (Tab). The hemolysin of the tested B. cereus strains was found to be thermolabile. The phospholipase-C activity withstood the temperature of 55 degrees C for a period of 10 minutes. The prepared specific antiserum, which inhibited the phsopholipase-C activity totally, had no effect on the hemolysis. Intradermal and enteropathogenic activities point to the presence of an enterotoxin. For detection of the enterotoxin, the skin-test in guinea pigs or rabbits seems to be most appropriate.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus cereus / Toxinas Bacterianas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: De Revista: Zentralbl Bakteriol Orig A Ano de publicação: 1977 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus cereus / Toxinas Bacterianas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: De Revista: Zentralbl Bakteriol Orig A Ano de publicação: 1977 Tipo de documento: Article