Effect of modeccin on rat liver ribosomes in vivo.
Biochim Biophys Acta
; 562(3): 495-503, 1979 May 24.
Article
em En
| MEDLINE
| ID: mdl-454613
ABSTRACT
1. Rat liver microsomes isolated at 6 and 12 h of poisoning with 3 x LD50 (0.3 microgram/100 g body wt.) of modeccin, the toxin of Adenia digitata, have a decreased capacity of protein synthesis in vitro. 2. A similar decrease of protein synthesis is observed with polysomes at 6 h of poisoning. Experiments with recombined ribosomal subunits demonstrate that this is due to inactivation of the 60 S ribosomal subunit. 3. At 6 h of poisoning there is a marked vesiculation and degranulation of the hepatocyte rough endoplasmic reticulum, which is completely fragmented at 24 h of poisoning. Hepatocyte mitochondria are swollen at 6 h and shrunk at 24 h of poisoning. 4. It is concluded that modeccin penetrates inside hepatocytes in vivo, and damages ribosomes in the same manner as it does in vitro. However, mitochondrial damage indicates that ribosomes may not be the only target of modeccin in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
/
Toxinas Biológicas
/
Lectinas
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1979
Tipo de documento:
Article