Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli.
J Bacteriol
; 152(1): 98-103, 1982 Oct.
Article
em En
| MEDLINE
| ID: mdl-6214548
ABSTRACT
The closely linked structural genes of phosphofructokinase (pfkA) and triosephosphate isomerase (tpi) of Escherichia coli were separately cloned onto plasmid pBR322. By gene dosage effects, transformed cells of E. coli C600 with these pBR322 hybrid plasmids showed 7- and 16-fold increases in the specific activities of phosphofructokinase and triosephosphate isomerase, respectively, over the specific activities in C600. Dried preparations of E. coli cells dosed with these genes showed appreciably high ATP-regenerating activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Trifosfato de Adenosina
/
Escherichia coli
/
Glicólise
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1982
Tipo de documento:
Article