Prostaglandin I2 synthesis and elevation of cyclic AMP levels in 3T3 fibroblasts.
Biochim Biophys Acta
; 618(3): 399-406, 1980 Jun 23.
Article
em En
| MEDLINE
| ID: mdl-6249378
ABSTRACT
Arachidonic acid and prostaglandin H2 elevate the levels of adenosine 3'5'-monophosphate (cyclic AMP) in Balb/c 3T3 fibroblasts. This effect was inhibited by 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid, an inhibitor of prostaglandin I2 synthase (Claesson, H.-E., Lindgren, J.A. and Hammarstr!om, S. (1977) FEBS Lett. 81, 415-418). After addition of arachidonic acid to 3T3 cultures, cellular cyclic AMP levels and growth medium concentrations of 6-ketoprostaglandin F1 alpha (degradation product of prostaglandin I2) were quantitatively determined. The stimulatory effect of exogenously-added prostaglandin I2 on cellular cyclic AMP levels was also determined. The results indicate that the endogenous production of prostaglandin I2 is sufficient to explain the stimulatory action of arachidonic acid on cyclic AMP formation in 3T3 fibroblasts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prostaglandinas
/
Epoprostenol
/
AMP Cíclico
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1980
Tipo de documento:
Article