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Cell free translation of chicken calcitonin messenger RNA.
FEBS Lett ; 157(1): 100-4, 1983 Jun 27.
Article em En | MEDLINE | ID: mdl-6862006
The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14 500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / RNA Mensageiro / Calcitonina Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 1983 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biossíntese de Proteínas / RNA Mensageiro / Calcitonina Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 1983 Tipo de documento: Article