Technetium-99m labeling of antibodies to cardiac myosin Fab and to human fibrinogen.
J Nucl Med
; 23(11): 1011-9, 1982 Nov.
Article
em En
| MEDLINE
| ID: mdl-7131084
ABSTRACT
We have developed a method of labeling biologically active labile macromolecules, such as human fibrinogen (HF) and anticardiac-myosin Fab (AM-Fab), with Tc-99m at neutral pH. This method uses dithionite reduction of pertechnetate and subsequent labeling, to test the method with acid-labile macromolecules. Complexes of diethylene triamine pentaacetic acid with macromolecules such as human fibrinogen (D-HF) and anticardiac-myosin Fab (D-AM-Fab) were labeled and utilized in in vitro and in vivo studies. In biodistribution studies, the Tc-99m D-HF had a two-component blood clearance (half-times 1 hr and 15 hr) and was 80--88% coagulable. The Tc-99m AM-Fab retained its immunoreactivity as tested by affinity chromatography; also during in vivo localization in experimental myocardial infarction. This labeling technique provides an easy and efficient approach to the Tc-99m labeling of other biologically active and acid-labile macromolecules.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibrinogênio
/
Fragmentos Fab das Imunoglobulinas
/
Miosinas
/
Tecnécio
/
Anticorpos
/
Miocárdio
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Nucl Med
Ano de publicação:
1982
Tipo de documento:
Article