Electron paramagnetic resonance investigation of X-irradiated poly(U), poly(A) and poly(A):poly(U): influence of hydration, packing and conformation on radical yield at 4 K.
Radiat Res
; 144(3): 251-7, 1995 Dec.
Article
em En
| MEDLINE
| ID: mdl-7494867
ABSTRACT
Powders and films of variably hydrated poly(U), poly(A) and poly(A)poly(U) were X-irradiated at 4 K. Spectra and free radical yields were acquired at 4 K using Q-band EPR spectroscopy. Evidence for electron transfer from the hydration layer to the RNA bases, supporting in part the damage transfer hypothesis of Gregoli et al. (Radiat. Res. 89, 238-254, 1985), is presented. Based on measurements of radical yield as a function of hydration, we propose that intermolecular packing and polymer conformation are dominant factors in determining free radical trapping ability in these polymers. Our annealing results indicate that increasing hydration facilitates intercluster combination reactions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Poli U
/
Poli A-U
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Radiat Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos