Nitric oxide synthesis in rat cardiac myocytes and fibroblasts.
Life Sci
; 55(14): 1101-8, 1994.
Article
em En
| MEDLINE
| ID: mdl-7522298
ABSTRACT
We investigated nitric oxide (NO) synthase activity in cultured neonatal rat cardiac myocytes and fibroblasts upon treatment with interleukin 1 beta (IL-1 beta) and lipopolysaccharide (LPS). Incubation of cardiac myocytes for 24 h with IL-1 beta or LPS caused a significant increase in NO and cGMP production. Simultaneous incubation of IL-1 beta with NG-monomethyl-L-arginine or transforming growth factor beta (TGF-beta) completely inhibited the IL-1 beta-induced NO and cGMP production in cardiac myocytes. In contrast, incubation of cardiac fibroblasts for 24 h with IL-1 beta or LPS showed no significant effect on NO or cGMP production. Addition of IL-1 beta decreased the beating rate of cardiac myocytes, but TGF-beta overcame that inhibition. These observations suggest the presence of iNOS in cardiac myocytes, which is an important regulator of contractile function of the heart.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibroblastos
/
Miocárdio
/
Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão