Histological, immunohistochemical, and ultrastructural findings in human corneas after photorefractive keratectomy.
J Refract Surg
; 11(3): 181-7, 1995.
Article
em En
| MEDLINE
| ID: mdl-7553088
ABSTRACT
BACKGROUND:
Four human corneas that had undergone photorefractive keratectomy (PRK) and subsequent penetrating keratoplasty were examined by means of light and electron microscopy in an attempt to detect possible causes for complications after PRK.METHODS:
Four eyes with a central corneal leukoma resulting from a previous PRK treatment underwent penetrating keratoplasty respectively 3 days, 3 months, 5 months, and 13 months after the refractive procedure. Different excimer laser instruments (Meditec MEL 50, Summit UV200, and VISX 20/20) had been used. The corneal buttons removed were submitted for light microscopy, electron microscopy and immunohistochemistry.RESULTS:
An immature (one to two layers, basement membrane anomalies) but continuous epithelium was present even in the 3-day specimen; between six and eight epithelial layers could be seen in the 13-month specimen, which still presented an undulated aspect of the otherwise normal basement membrane. A continuous acellular collagen layer underlying the epithelium of the ablated area was detected in the superficial stroma of all examined corneas. No Descemet's membrane or endothelial alterations could be seen.CONCLUSIONS:
Despite recovery of a continuous epithelial layer as early as 3 days after PRK, abnormalities of both epithelium and superficial stroma could be detected in all specimens, including the one obtained 13 months after the refractive procedure.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Erros de Refração
/
Ceratectomia Fotorrefrativa
/
Córnea
/
Opacidade da Córnea
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Refract Surg
Assunto da revista:
OFTALMOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália