Genotypic analysis of multiple loci in somatic cells by whole genome amplification.
Nucleic Acids Res
; 23(17): 3488-92, 1995 Sep 11.
Article
em En
| MEDLINE
| ID: mdl-7567460
ABSTRACT
To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA de Neoplasias
/
Neoplasias Esofágicas
/
Adenocarcinoma
/
Reação em Cadeia da Polimerase
Limite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos