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Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus.
Macedo, M L; Fernandes, K V; Sales, M P; Xavier-Filho, J.
Afiliação
  • Macedo ML; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Braz J Med Biol Res ; 28(2): 183-90, 1995 Feb.
Article em En | MEDLINE | ID: mdl-7581039
Vicilins (7S storage proteins) from seeds of cowpea (Vigna unguiculata) cultivars which are susceptible or resistant to the bruchid beetle C. maculatus were purified by size-exclusion and ion-exchange chromatography. The vicilins were partially characterized by polyacrylamide gel electrophoresis under both denaturing and nondenaturing conditions, by Western blotting and by amino acid analysis. The variant vicilins from C. maculatus-resistant seeds do not differ appreciably from vicilins from susceptible seeds by these criteria except that they are more strongly bound to DEAE-Sepharose, suggesting differences in charge between the various molecules.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Sementes / Besouros Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Brasil
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Sementes / Besouros Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 1995 Tipo de documento: Article País de afiliação: Brasil