Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies.
Infect Immun
; 63(11): 4476-80, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-7591088
ABSTRACT
We have attempted to express the Helicobacter pylori vacuolating cytotoxin in Escherichia coli. Although the 95-kDa VacA polypeptide was expressed abundantly, it completely lacked any biological activity. In addition, this material failed to induce neutralizing antibodies after immunization of rabbits. In contrast, highly purified high-molecular-mass cytotoxin from the supernatant of H. pylori cultures was active in a HeLa cell assay and effectively induced a neutralizing response in rabbits. Neutralizing sera were shown to contain a high proportion of antibodies which recognized conformational epitopes found only on the native toxin. The data indicate that toxin-neutralizing epitopes are conformational and that potential vaccines based on the cytotoxin may benefit from the use of the intact molecule.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Helicobacter pylori
/
Citotoxinas
/
Anticorpos Antibacterianos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália