Pasteurella haemolytica leukotoxin-induced synthesis of eicosanoids by bovine neutrophils in vitro.
J Leukoc Biol
; 56(5): 644-9, 1994 Nov.
Article
em En
| MEDLINE
| ID: mdl-7964171
ABSTRACT
Exposure of isolated bovine neutrophils to partially purified Pasteurella haemolytica leukotoxin caused increased synthesis of leukotriene B4 (LTB4) but not thromboxane B2 (TXB2) from endogenous arachidonic acid. Synthesis of LTB4 was closely correlated with leukotoxin-induced neutrophil lysis. At low toxin concentrations, LTB4 production lagged behind leukotoxin-induced neutrophil lysis over a 3-h period. The neutralizing monoclonal antileukotoxin antibody MM601 neutralized both leukotoxin-induced neutrophil lysis and LTB4 synthesis. Both leukotoxin-induced neutrophil lysis and LTB4 synthesis were Ca(2+)-dependent. When leukotoxin-induced LTB4 synthesis from exogenous arachidonic acid was examined, significant LTB4 synthesis occurred at 5 min of leukotoxin exposure, which was before leukotoxin-induced lysis developed. Leukotoxin-induced LTB4 synthesis from endogenous arachidonic acid appears to require leukotoxin-induced plasma membrane damage (occurring during neutrophil lysis), whereas LTB4 synthesis from exogenous arachidonic acid is initiated rapidly and occurs in the absence of plasma membrane damage.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mannheimia haemolytica
/
Leucotrieno B4
/
Citotoxinas
/
Exotoxinas
/
Neutrófilos
Limite:
Animals
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
1994
Tipo de documento:
Article