[The isolation and purification of porcine interferon-gamma]. / Poluchenie i ochistka svinogo gamma-interferona.

ABSTRACT

Porcine immune interferon (IF-gamma) was generated by stimulation with bacterial lectins. Physicochemical analysis showed porcine IF-gamma to be a glycoprotein with an isoelectric point around 8.4-8.8 and an apparent molecular weight of 28000 to 30000. A purification process used in this work included sequential steps of precipitation with ammonium sulphate and chromatographic separation on GPG-glass, con A-Sepharose and biogel P-100. This purification process gave a preparation of IF-gamma with specific activity of around 7 x 10(4) units per mg of protein.