Over-expression of the yeast ATP synthase subunit D in Escherichia coli: use of polyclonal antibodies directed against recombinant subunit D.
Biochem Biophys Res Commun
; 200(2): 877-83, 1994 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-8179622
ABSTRACT
The yeast ATP synthase subunit d was over-expressed in E. coli and formed inclusion bodies. It was purified by solubilization in urea and slow removal of the urea by stepwise dialysis in the presence of a non-ionic detergent. The resulting soluble subunit d was used to prepare polyclonal antibodies. Blots of yeast mitochondrial proteins were probed with these antibodies. The strain disrupted in ATP4 gene encoding the subunit 4 displayed only 8% of the wild type subunit d. Antibodies against subunit d did not inhibit the wild type ATPase activity.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
ATPases Translocadoras de Prótons
/
Escherichia coli
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França