Expression of PP2A B regulatory subunit beta isotype in rat testis.
FEBS Lett
; 324(1): 71-5, 1993 Jun 07.
Article
em En
| MEDLINE
| ID: mdl-8389301
ABSTRACT
We isolated a rat cDNA encoding part of the beta-isotype of the B regulatory subunit (BR beta) of protein phosphatase 2A (PP2A). The isolated cDNA encoded the region corresponding to amino acids positions 8(R) to 177(N) of human BR beta. The identities of the nucleotide and amino acid sequences of the rat and human BR beta s were 95.7% and 100%, respectively. The BR beta mRNA was specifically expressed in rat brain and testis, the lengths of mRNAs in these two organs being different. In the testis, the BR beta mRNA was first detected 40 days after birth, increasing gradually thereafter, and was expressed specifically in elongated spermatids, while mRNA of the alpha-isotype (BR alpha) was expressed equally in all spermatogenic cells. After meiosis, round spermatids change morphologically to elongated spermatids. BR beta may regulate the activity of the PP2A catalytic subunit in spermatids, and be involved in spermatogenic maturation, especially spermatid elongation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermátides
/
Espermatócitos
/
Testículo
/
Fosfoproteínas Fosfatases
/
Isoenzimas
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão