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Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium.
Moukha, S M; Wösten, H A; Mylius, E J; Asther, M; Wessels, J G.
Afiliação
  • Moukha SM; Laboratoire de Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy, Parc Scientifique et Technologique, Marseille, France.
J Bacteriol ; 175(11): 3672-8, 1993 Jun.
Article em En | MEDLINE | ID: mdl-8501073
ABSTRACT
Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peroxidases / RNA Mensageiro / Fungos Idioma: En Revista: J Bacteriol Ano de publicação: 1993 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peroxidases / RNA Mensageiro / Fungos Idioma: En Revista: J Bacteriol Ano de publicação: 1993 Tipo de documento: Article País de afiliação: França